DMTR-biotin-PEG2-amine

DMTR-biotin-PEG2-amine

This product is a high-purity, heterobifunctional biotinylation reagent with a DMTr-protected amine and a biotin moiety specifically linked by a PEG2 spacer. The PEG2 improves solubility and flexibility, while the DMTr group allows selective deprotection for orthogonal, precise bioconjugation and assay development.

Key Features and Benefits:

• Biotin Moiety: Enables high-affinity binding to streptavidin or avidin-based systems for detection, purification, and immobilization.

• DMTr-Protected Amine: Allows controlled deprotection for orthogonal or stepwise functionalization.

• PEG2 Spacer: Improves solubility, flexibility, and accessibility of the reactive groups.

• Heterobifunctional Design: Supports sequential conjugation strategies for complex biomolecule assembly.

• High Purity: Supplied at >95% purity for reliable and reproducible performance.

Applications:

• Biotinylation of Biomolecules: Attach biotin to peptides, oligonucleotides, proteins, or small molecules.

• Orthogonal Bioconjugation: Perform stepwise labeling with selective DMTr deprotection.

• Assay and Diagnostic Development: Prepare biotin-labeled probes for ELISA, western blotting, and biosensors.

• Affinity Purification: Create biotinylated ligands for streptavidin-based pull-down assays.

• Surface Functionalization: Immobilize biomolecules on streptavidin-coated microplates, beads, or sensors.

Storage and Handling:

• Storage: Store at -20°C, in a dry, dark environment.

• Handling: Use anhydrous solvents such as DMSO or DMF. Avoid exposure to moisture to preserve DMTr-protected functionality.

This product offers a balanced design of biotin affinity, PEG2 flexibility, and orthogonal reactivity, making it an especially excellent tool for controlled bioconjugation and assay development.