Biotin-ONP

Biotin-ONP CAS:33755-53-2 is a biotinylated reagent commonly used in bioconjugation and protein modification applications. The reagent consists of a biotin group and an o-nitrophenyl (ONP) group connected by a linker.

The biotin group, a vitamin H derivative, is widely used in bioconjugation applications because of its strong and selective binding to avidin or streptavidin. The ONP group is a chromogenic group that can be easily detected by UV-visible spectroscopy, which makes it a useful reagent for quantifying the amount of biotinylated protein in a sample.

Biotin-ONP is commonly used in the modification of proteins, peptides, and other biomolecules. It can be used to attach biotin to target molecules, which can be used for detection, purification, or immobilization purposes. The ONP group can also be used to quantify the amount of biotinylated protein in a sample.

Biotin-ONP is a yellow to orange powder that is soluble in a range of organic solvents, such as DMSO, DMF, or ethanol. It should be stored at -20°C in a dry and dark place to maintain its stability and quality.