Biotin-SLC-NHS Ester

Biotin-SLC-NHS Ester

This product is a sulfo-long-chain biotin reagent featuring an NHS ester for rapid and efficient amine conjugation. The long-chain, sulfonated spacer enhances water solubility, reduces steric hindrance, and improves accessibility, making it especially ideal for biotinylation of proteins, antibodies, and biomolecules in aqueous systems.

Key Features and Benefits:

• Biotin Moiety: Provides strong avidin/streptavidin binding for detection and purification.

• NHS Ester: Couples efficiently with primary amines under mild conditions.

• SLC Spacer: Long-chain, sulfonated PEG-like structure improves solubility and accessibility.

• High Affinity + Solubility: Enables efficient labeling in aqueous buffers.

• High Purity: Ensures reproducibility in biotinylation workflows.

Applications:

• Protein Labeling: Biotinylates antibodies, enzymes, and other amine-containing proteins.

• Aqueous Bioconjugation: Ideal for reactions performed in physiological buffers.

• Diagnostics: Used in ELISA, Western blotting, and immunoprecipitation assays.

• Purification: Facilitates streptavidin-based capture and affinity separations.

Storage and Handling:

• Storage: Store at –20 °C in a tightly sealed, light- and moisture-protected container.

• Handling: Use freshly prepared solutions; avoid repeated freeze–thaw cycles.

This product combines high-affinity biotin binding with sulfonated spacer solubility, offering reliable and efficient protein labeling for biochemical assays and conjugation.