Biotinyl tyramide

Biotinyl tyramide

This product is a biotin derivative widely used in tyramide signal amplification (TSA) assays. It specifically enables covalent deposition of biotin at sites of peroxidase activity, significantly enhancing sensitivity in immunohistochemistry, in situ hybridization, and ELISA applications.

Key Features and Benefits

• Biotin Moiety: Provides strong binding affinity to streptavidin and avidin systems.

• Tyramide Core: Enables enzymatic deposition via peroxidase activity.

• Signal Amplification: Increases detection sensitivity in low-abundance targets.

• Broad Compatibility: Works with immunohistochemistry, in situ hybridization, and blotting assays.

• High Purity: Ensures reliable and reproducible signal amplification.

Applications

• Immunohistochemistry: Enhances detection of proteins with TSA-based amplification.

• In Situ Hybridization: Improves signal clarity for nucleic acid detection.

• ELISA and Blotting: Boosts sensitivity for low-expression biomarkers.

• Probe Development: Serves as a biotinylated tool for enzymatic labeling workflows.

Storage and Handling

• Storage: Keep at –20 °C in a sealed, light- and moisture-protected container.

• Handling: Avoid repeated freeze–thaw cycles; prepare fresh working solutions as needed.

This product combines enzymatic deposition with biotin–streptavidin affinity, specifically providing a powerful reagent for sensitive detection and amplification in biological assays.