DBCO-NH-PEG12-Biotin

DBCO-NH-PEG12-Biotin

This product is a heterobifunctional linker containing a dibenzocyclooctyne (DBCO) group, a PEG12 spacer, a secondary amine, and a biotin tag. The DBCO moiety enables ultrafast, copper-free strain-promoted azide–alkyne cycloaddition (SPAAC) with azides, while the biotin provides strong affinity binding to streptavidin, avidin, and NeutrAvidin. The PEG12 chain enhances solubility, reduces steric hindrance, and improves biotin accessibility, making this reagent especially effective for dual-functional bioconjugation.

Key Features and Benefits

• DBCO Group: Supports rapid, copper-free SPAAC click reactions with azides.

• Biotin Tag: Ensures strong and specific binding to streptavidin and avidin systems.

• PEG12 Spacer: Provides solubility, flexibility, and extended reach.

• Dual Reactivity: Combines bioorthogonal click chemistry with affinity capture.

• High Purity: Produced to ≥95% purity for reproducible applications.

Applications

• Protein and Antibody Labeling: Enables dual labeling with click chemistry and biotin tagging.

• Affinity Capture: Immobilizes biomolecules on streptavidin-coated beads, chips, or surfaces.

• Probe Development: Builds multifunctional probes for imaging and diagnostics.

• Drug Delivery: Serves as a dual-functional linker in targeted therapeutic systems.

Storage and Handling

• Storage: Store at –20 °C in a sealed, desiccated container.

• Handling: Prepare fresh solutions before use; avoid repeated freeze–thaw cycles.

This product combines copper-free click reactivity with strong biotin binding on a PEG12 backbone, specifically providing a versatile tool for advanced conjugation and affinity-based applications.