DBCO-Biotin

DBCO-Biotin

This product is a heterobifunctional reagent combining a dibenzocyclooctyne (DBCO) group with biotin. It specifically enables copper-free click chemistry with azides and provides strong streptavidin/avidin affinity for capture, detection, and imaging.

Key Features and Benefits

• Dual Functionality: DBCO enables copper-free click with azides; biotin provides streptavidin binding.

• Copper-Free Click Chemistry: Safe for live-cell and in vivo use, avoiding cytotoxic catalysts.

• High Specificity: Rapid, selective conjugation under physiological conditions.

• Affinity Tag: Biotin allows pull-down, detection, and immobilization assays.

• High Purity: Ensures reproducibility in bioconjugation and diagnostic workflows.

Applications

• Affinity Capture: Binds azide-modified biomolecules via streptavidin/avidin systems.

• Diagnostics: Supports detection platforms and assay development.

• Bioorthogonal Labeling: Enables imaging and tracking in cells and tissues.

• Proteomics: Facilitates pull-down assays and biomolecule immobilization.

• Drug Delivery: Used in targeted delivery studies and conjugate design.

Storage and Handling

• Storage: Store at –20 °C in a sealed, dry, and light-protected container.

• Handling: Use anhydrous solvents such as DMSO; avoid prolonged exposure to moisture.

This product integrates a DBCO click handle with biotin affinity, specifically providing efficient copper-free conjugation and reliable capture for bioconjugation and diagnostic applications.