Biotin-PEG6-NHS Ester

Biotin-PEG6-NHS Ester

This product is a high-purity, amine-reactive biotinylation reagent consisting of a biotin moiety linked to an NHS ester through a hydrophilic PEG6 spacer. The PEG6 chain specifically improves water solubility, reduces steric hindrance, and enhances biotin accessibility for binding to streptavidin or avidin. The NHS ester efficiently reacts with primary amines under mild, aqueous conditions, making this reagent suitable for labeling proteins, peptides, antibodies, and other amine-containing biomolecules.

Key Features

• Biotin moiety for strong and specific binding to streptavidin/avidin.

• PEG6 spacer for improved solubility and reduced steric hindrance.

• NHS ester group for rapid, efficient conjugation to primary amines.

• Suitable for aqueous and mild reaction conditions.

Applications

• Protein, peptide, and antibody biotinylation.

• Preparation of biotinylated probes for detection assays.

• Surface modification for biosensors and affinity purification.

• Conjugation in ELISA, western blotting, and immunoprecipitation workflows.

Storage and Handling

• Store at –20 °C, protected from moisture and light.

• Use anhydrous solvents for optimal stability.

• Avoid repeated freeze–thaw cycles.

This product is a versatile biotinylation reagent offering high solubility, low steric interference, and efficient amine conjugation. It is especially used in biomolecule labeling, detection, and affinity-based purification applications.