Biotin-PEG3-NHS Ester

Biotin-PEG3-NHS Ester

This product is a high-purity biotinylation reagent featuring an NHS ester for rapid and efficient conjugation with primary amines. The PEG3 spacer enhances solubility, reduces steric hindrance, and improves biotin accessibility, making it especially suitable for labeling proteins, peptides, and other biomolecules in aqueous and organic systems.

Key Features and Benefits

• NHS Ester – Reacts efficiently with lysine residues or other primary amines.

• Biotin Moiety – Provides strong and specific binding to streptavidin or avidin.

• PEG3 Spacer – Improves water solubility and reduces steric interference.

• Versatile Biotinylation – Compatible with proteins, peptides, antibodies, and nanoparticles.

• High Purity – Supplied at >95% purity for consistent performance.

Applications

• Protein and peptide biotinylation

• Antibody labeling for ELISA, western blot, and flow cytometry

• Streptavidin–biotin capture and detection assays

• Surface modification of beads, nanoparticles, or biosensors

Storage & Handling

• Storage: Store at –20 °C, dry, and protected from light

• Use: Dissolve immediately before use in anhydrous DMSO or DMF. Perform reactions in pH 7.0–8.5 buffer for optimal NHS ester activity.

This product is a biotinylation reagent with an NHS ester for amine conjugation, linked via PEG3 to enhance solubility and reduce steric hindrance, especially ideal for labeling biomolecules in binding and detection assays.