Biotin-PEG24-NHS Ester

Biotin-PEG24-NHS Ester

This product is a biotinylation reagent with a biotin tag, a PEG24 spacer, and an NHS ester group. The biotin specifically binds tightly to streptavidin, avidin, and NeutrAvidin systems and the NHS ester reacts efficiently with primary amines to form stable amide bonds. The long PEG24 spacer specifically improves solubility, enhances biotin accessibility, and reduces steric hindrance for demanding conjugation workflows.

Key Features and Benefits

• Biotin Tag: Provides strong, specific binding to streptavidin and avidin.

• NHS Ester Group: Couples rapidly with amines for stable amide bonds.

• PEG24 Spacer: Adds excellent solubility, flexibility, and extended spacing.

• High Accessibility: Minimizes steric effects in biomolecule labeling.

• High Purity: Produced to ≥95% purity for consistent results.

Applications

• Protein and Antibody Labeling: Enables efficient biotinylation for detection and purification.

• Affinity Capture: Immobilizes biomolecules on streptavidin-coated surfaces or beads.

• Assay Development: Supports ELISA, Western blot, and immunohistochemistry.

• Drug Delivery: Functions as a biotin linker in therapeutic systems.

Storage and Handling

• Storage: Store at –20 °C in a sealed, desiccated container.

• Handling: Dissolve freshly in dry DMSO or DMF; avoid repeated freeze–thaw cycles.

This product combines biotin affinity, NHS ester reactivity, and long PEG spacing, specifically providing a reliable reagent for bioconjugation and assay development.