Biotin-PEG2-amine

Biotin-PEG2-amine

Biotin-PEG2-amine is a high-purity heterobifunctional PEG linker with a biotin group for strong streptavidin/avidin binding and a primary amine for versatile conjugation. The PEG2 spacer enhances water solubility, flexibility, and reduces steric hindrance, enabling efficient bioconjugation in both aqueous and organic systems. This design is widely used in assay development, affinity purification, imaging, and targeted delivery applications.

Key Features and Benefits

• Biotin Group: Provides high-affinity binding to streptavidin, neutravidin, and avidin proteins.

• Primary Amine: Couples efficiently with NHS esters, activated acids, and other electrophiles.

• PEG2 Spacer: Improves solubility, flexibility, and accessibility of reactive groups.

• Heterobifunctional Design: Supports orthogonal and sequential modification strategies.

• High Purity: Supplied at >95% purity for reliable and reproducible performance.

Applications

• Affinity Purification: Immobilize or capture biotin-binding proteins and complexes.

• Protein and Peptide Labeling: Introduce biotin handles while coupling to other biomolecules.

• Diagnostic Assays: Develop biotin-labeled probes for ELISA, Western blot, and other detection formats.

• Targeted Delivery Systems: Link biotinylated ligands to delivery vehicles for targeted binding.

• Imaging Probes: Prepare biotin-based imaging agents for microscopy or in vivo studies.

Storage and Handling

• Storage: Store at –20 °C, dry, and protected from light.

• Handling: Use anhydrous solvents such as DMF or DMSO; avoid prolonged exposure to moisture to maintain reactivity.

Biotin-PEG2-amine combines high-affinity biotin binding with amine-based conjugation in a hydrophilic PEG2 framework, specifically offering versatility for bioconjugation in research, diagnostics, and targeted delivery.