TCO-PEG4-CH2 acid

TCO-PEG4-CH2 Acid

This high-purity PEG linker has a TCO for rapid, catalyst-free IEDDA cycloaddition with tetrazines and a terminal carboxylic acid for amine coupling. The PEG4 spacer specifically enhances solubility, flexibility, and reduces steric hindrance for efficient, orthogonal bioconjugation in aqueous and organic systems.

Key Features and Benefits

• TCO Group – Enables ultra-fast, catalyst-free tetrazine ligation for bioorthogonal labeling.

• Carboxylic Acid – Couples efficiently to primary amines via carbodiimide or other activation chemistries.

• PEG4 Spacer – Enhances solubility, flexibility, and accessibility of reactive groups.

• Orthogonal Reactivity – Allows sequential, site-specific modifications without cross-reactivity.

• High Purity – Supplied at >95% purity for reproducible results.

Applications

• Bioorthogonal labeling of proteins, peptides, and nucleic acids

• Antibody–drug conjugate (ADC) linker chemistry

• Multifunctional imaging and diagnostic probe synthesis

• Nanoparticle and biomaterial surface functionalization

Storage and Handling

• Storage: Store at –20 °C, dry, and protected from light

• Handling: Use anhydrous solvents such as DMSO or DMF; avoid prolonged exposure to moisture to preserve reactivity

This product combines tetrazine ligation via TCO with a reactive carboxylic acid, connected by a PEG4 spacer for solubility and flexibility, making it especially ideal for precise, orthogonal bioconjugation.