TCO-PEG4-amido maleimide

TCO-PEG4-Amido Maleimide

This high-purity heterobifunctional PEG linker has a trans-cyclooctene (TCO) group for rapid, catalyst-free inverse electron-demand Diels–Alder (IEDDA) cycloaddition with tetrazines and a maleimide specifically for selective thiol coupling. The PEG4 spacer enhances solubility, flexibility, and reduces steric hindrance, enabling efficient, orthogonal bioconjugation in aqueous and organic systems.

Key Features and Benefits

• TCO Group – Enables ultra-fast, catalyst-free tetrazine ligation for bioorthogonal labeling.

• Maleimide Group – Reacts selectively with thiols via Michael addition under mild conditions.

• PEG4 Spacer – Improves solubility and accessibility of reactive groups while reducing steric hindrance.

• Orthogonal Reactivity – Supports sequential, site-specific modifications without cross-reactivity.

• High Purity – Supplied at >95% purity for consistent, reproducible results.

Applications

• Dual-functional linker for orthogonal conjugation

• Protein, peptide, and antibody modification

• Antibody–drug conjugate (ADC) linker chemistry

• Multifunctional imaging and diagnostic probe synthesis

Storage and Handling

• Storage: Store at –20 °C, dry, and protected from light

• Handling: Use anhydrous solvents such as DMSO or DMF; avoid prolonged moisture exposure to preserve reactivity

Summary: TCO-PEG4-amido maleimide combines tetrazine ligation via TCO with thiol–maleimide conjugation, linked by a PEG4 spacer for solubility and flexibility, making it ideal for precise, orthogonal bioconjugation.