Mal-PEG3-azide

Mal-PEG3-azide

Mal-PEG3-azide is a high-purity heterobifunctional PEG linker with a maleimide group for thiol-specific conjugation and an azide group for copper-catalyzed (CuAAC) or strain-promoted (SPAAC) click chemistry. The PEG3 spacer enhances solubility, flexibility, and reduces steric hindrance, enabling efficient bioconjugation in both aqueous and organic systems. This design is especially ideal for orthogonal, stepwise conjugations where thiol and azide chemistries are used sequentially or in combination.

Key Features and Benefits

• Maleimide Group: Selectively reacts with thiols to form stable thioether bonds.

• Azide Group: Reacts with alkynes via CuAAC or SPAAC for bioorthogonal conjugation.

• PEG3 Spacer: Improves water solubility, flexibility, and accessibility of reactive groups.

• Heterobifunctional Design: Enables orthogonal, sequential modification strategies.

• High Purity: Supplied at >95% purity for reproducible and consistent results.

Applications

• Stepwise Bioconjugation: Combine thiol labeling and click chemistry in one molecule.

• Protein and Peptide Modification: Introduce azide handles to thiol-containing biomolecules.

• Nanoparticle and Surface Functionalization: Attach thiol-reactive and clickable moieties to surfaces.

• Drug Delivery Systems: Create multifunctional linkers for targeted payload delivery.

• Imaging and Diagnostic Probes: Prepare dual-labeled probes using orthogonal chemistries.

Storage and Handling

• Storage: Store at –20 °C, dry, and protected from light.

• Handling: Use anhydrous solvents such as DMF or DMSO; avoid prolonged moisture exposure to maintain reactivity.

This product combines thiol reactivity and click chemistry in a hydrophilic PEG3 framework, offering versatility specifically for orthogonal bioconjugation in therapeutics, diagnostics, and materials science.