DBCO-NH-PEG2-Mal

DBCO-NH-PEG2-Mal

This product is a high-purity heterobifunctional PEG linker containing a dibenzocyclooctyne (DBCO) group for strain-promoted azide–alkyne cycloaddition (SPAAC) click chemistry and a maleimide group for thiol-specific conjugation. The short PEG2 spacer improves solubility and provides flexibility while maintaining compactness, making it especially efficient for dual bioconjugation in both aqueous and organic systems.

Key Features and Benefits

• DBCO Group – Enables copper-free SPAAC click chemistry with azide-containing molecules.

• Maleimide Group – Selectively reacts with thiols for stable thioether bond formation.

• PEG2 Spacer – Enhances solubility, flexibility, and minimizes steric hindrance.

• Orthogonal Reactivity – Supports sequential or site-specific dual conjugation strategies.

• High Purity – Supplied at >95% purity for reliable and reproducible performance.

Applications

• Dual-functional bioconjugation of proteins, peptides, and antibodies

• Antibody–drug conjugate (ADC) development

• Multifunctional probe and biosensor design

• Nanoparticle and hydrogel surface modification

Handling

• Storage: Store at –20 °C, dry, and protected from light

• Use: Dissolve in anhydrous solvents such as DMSO or DMF. Handle maleimide under inert or buffered conditions (pH 6.5–7.5) to prevent hydrolysis.

This product combines a DBCO group for SPAAC click chemistry with a maleimide for thiol-specific conjugation, connected by a PEG2 spacer for solubility and flexibility, enabling especially efficient orthogonal dual labeling strategies.