Biotin-NHS Ester

Catalog #:	AP10489
Chemical Formula:	C₁₄H₁₉N₃O₅S
Molecular Weight:	341.38
CAS:	35013-72-0
Purity:	95%
Availability:	In Stock
SDS:	Download

Unit	Price (USD)
250mg	$80.00	Request quote
500mg	$120.00	Request quote
1g	$160.00	Request quote
2 g	$240.00	Request quote
5 g	$750.00	Request quote

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Biotin-NHS Ester

This product is an amine-reactive biotinylation reagent containing an NHS ester group. It specifically enables efficient labeling of proteins, peptides, and other amine-containing molecules, offering strong biotin–streptavidin binding and high sensitivity in detection.

Key Features and Benefits

Biotin Moiety: Provides strong, specific binding to streptavidin and avidin.
NHS Ester: Couples efficiently with primary amines to form stable amide bonds.
High Reactivity: Ensures fast labeling under mild conditions.
Versatile Use: Suitable for proteins, peptides, antibodies, and small molecules.
High Purity: Guarantees reproducibility in bioconjugation workflows.

Applications

Bioconjugation: Labels proteins, peptides, and nucleic acids with biotin.
Probe Development: Builds biotinylated probes for diagnostics and assays.
Detection Assays: Supports ELISA, Western blotting, and immunoprecipitation.
Affinity Purification: Enables capture and isolation of biotinylated molecules.

Storage and Handling

Storage: Store at –20 °C in a sealed, light- and moisture-protected container.
Handling: Dissolve in anhydrous DMSO or DMF; prepare fresh solutions for best results.

This product integrates amine-reactive chemistry with strong biotin affinity, specifically providing a robust reagent for biotinylation and detection workflows.

Synonyms: Biotin-NHS;(+)-Biotin N-hydroxysuccinimide ester;Biotin NHS;NHS-Biotin;Biotin N-Hydroxysuccinimide Ester;D-Biotinyl-osu;Biotin-OSu;BHSE;2,5-Dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate;N-Succinimidyl D-biotinate;(+)-Biotin-NHS ester;N-Hydroxysuccinimidobiotin;Biotinyl N-hydroxysuccinimide Ester;C14H19N3O5S;Biotin-NHS ester;Biotine-OSu;1-((5-(2-Oxohexahydro-1H-thieno(3,4-d)imidazol-4-yl)pentanoyl)oxy)-2,5-pyrrolidinedione;D-biotin-N-hydroxysuccinimide ester;(2,5-dioxopyrrolidin-1-yl) 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate;2,5-Dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate;2,5-dioxopyrrolidin-1-yl 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoate;succinimidyl d-biotin;biotin succinimidyl ester;N-(D-Biotinyloxy)succinimide;D-Biotin N-Succinimidyl Ester;(+)-Biotin-NHS;BIOTIN-NHS;d-biotin n-hydroxysuccinimide ester;2,5-Dioxo-1-pyrrolidinyl 5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoate;( )-Biotin N-hydroxysuccinimide ester;2,5-Pyrrolidinedione,1-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]oxy]-;2,5-Dioxopyrrolidin-1-yl 5-((4S)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate;2,5-Dioxopyrrolidin-1-yl-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate;5-(2-Oxo-hexahydro-thieno[3,4-d]imidazol-6-yl)-pentanoic acid 2,5-dioxo-pyrrolidin-1-yl ester;6aR-Hexahydro-2-oxo-2,5-dioxo-1-pyrrolidinyl ester-1H-thieno[3aS,4-d]imidazole-4S-pentanoic acid

Related Products

CAT#	Name	M.W.	Purity	Price
AP10496	Biotin-SLC-NHS Ester	524.68	95%	$340.00–$1460.00
AP10494	Biotin-LC-LC-NHS Ester	567.70	95%	$140.00–$890.00