Biotin-LC-LC-NHS Ester

Biotin-LC-LC-NHS Ester

This product is a biotinylation reagent featuring a biotin group, an NHS ester for amine coupling, and a long-chain LC–LC spacer specifically for reduced steric hindrance. The extended PEG-like spacing specifically improves solubility and accessibility, making it ideal for protein labeling, probe development, and bioconjugation.

Key Features and Benefits:

• Biotin Moiety: Provides strong avidin/streptavidin binding.

• NHS Ester: Efficiently couples with primary amines under mild conditions.

• LC–LC Spacer: Reduces steric hindrance and enhances solubility.

• Long Reach: Improves biotin accessibility in complex biomolecular systems.

• High Purity: Ensures reproducibility in labeling workflows.

Applications:

• Protein Labeling: Biotinylates antibodies, enzymes, and other amine-containing biomolecules.

• Probe Development: Builds biotinylated probes for diagnostics and imaging.

• Bioconjugation: Couples biotin to peptides, polymers, and nanoparticles.

• Drug Delivery: Provides long, flexible spacers for improved biotin-targeted systems.

Storage and Handling:

• Storage: Store at –20 °C in a sealed, moisture- and light-protected container.

• Handling: Use freshly prepared solutions; NHS ester is moisture-sensitive.

This product combines biotin binding with amine-reactive NHS chemistry and long-chain flexibility, making it especially effective for reducing steric hindrance in labeling.