Methyltetrazine-PEG4-amine HCl Salt

Methyltetrazine-PEG4-amine HCl salt

This product is a heterobifunctional PEG4 linker with a methyltetrazine for rapid inverse-electron-demand Diels–Alder (IEDDA) click chemistry and a primary amine provided as a hydrochloride salt for enhanced solubility and stability. The PEG4 spacer offers hydrophilicity and flexibility, making it especially useful for orthogonal bioconjugation, probe design, and therapeutic applications.

Key Features and Benefits:

• Methyltetrazine Group: Reacts rapidly with strained alkenes such as TCO and BCN.

• Amine Group (HCl Salt): Stable, water-soluble form that couples efficiently with carboxyls and NHS esters.

• PEG4 Spacer: Provides compact hydrophilic spacing and reduced steric hindrance.

• High Purity: Ensures reproducibility in bioconjugation and therapeutic workflows.

• Dual Reactivity: Supports orthogonal strategies combining IEDDA click chemistry and amide coupling.

Applications:

• Bioorthogonal Labeling: Enables ultrafast tetrazine–TCO ligation for in vitro and in vivo studies.

• Bioconjugation: Facilitates dual-functional labeling of proteins, peptides, and biomolecules.

• Probe Development: Useful in fluorescent, radiolabeled, or affinity probe synthesis.

• Drug Delivery: PEGylated linker improves solubility, circulation, and targeted delivery.

Storage and Handling:

• Storage: Store at –20 °C in a sealed, light-protected container.

• Handling: Use aqueous buffers or anhydrous solvents (e.g., DMF, DMSO); avoid prolonged light exposure to preserve tetrazine reactivity.

This product combines ultrafast tetrazine click chemistry with amine reactivity in a compact PEG chain, making it an especially excellent reagent for advanced conjugation, probe development, and therapeutic applications.