4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol

4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol

This product is an aromatic tetrazine derivative featuring a phenolic hydroxyl group and a methyl-substituted tetrazine ring. The tetrazine moiety undergoes rapid inverse-electron-demand Diels–Alder (IEDDA) reactions with strained alkenes such as TCO or BCN, while the phenolic group provides a versatile handle for further functionalization, making this compound highly valuable in bioorthogonal chemistry and probe development.

Key Features and Benefits:

• Tetrazine Moiety: Enables ultrafast IEDDA click chemistry with strained alkenes.

• Phenolic Hydroxyl: Offers a reactive site for further derivatization or conjugation.

• Methyl Substitution: Enhances stability and reactivity in click reactions.

• High Purity: Ensures reproducibility in sensitive chemical biology workflows.

• Compact Design: Useful as a building block in small-molecule probes and linkers.

Applications:

• Bioorthogonal Chemistry: Enables rapid ligation with TCO/BCN for in vitro and in vivo labeling.

• Probe Development: Serves as a precursor for fluorescent, radiolabeled, or affinity probes.

• Bioconjugation: Supports site-specific protein, peptide, or antibody modification.

• Synthetic Chemistry: Intermediate for advanced tetrazine-based conjugates.

Storage and Handling:

• Storage: Store at –20 °C, protected from light and moisture.

• Handling: Use anhydrous solvents (e.g., DMSO, DMF) and avoid prolonged light exposure to maintain tetrazine stability.

This product provides fast bioorthogonal reactivity and a versatile phenolic group, making it an especially excellent intermediate for conjugation, probe development, and advanced material applications.